THC Testing

CTK CannabisTesting Kits Information

THC testing with TLC Supply's CTK test kits are performed using “Thin Layer Chromatography”. These testing kits are designed specifically for testing Marijuana sometimes called HPTLC. It is a patented and registered product, and was certified by the University of Leiden.This kit will provide you with visual color indicators for the THC, THCV, CBG, CBNV, CBD substances and more in Medical Marijuana testing. There is testing for estimated THC % with visual indicators, and testing for acids and poisons. This THC testing kit is a good step in providing a balanced product that provides a way for growers and product producers to analyze there products during production to produce these products, and an 

alternative to laboratory testing.

Here is some information that 

will come with the kit: 

You get step by step directions with pictures of each step being taken. You get all the little reaction chambers and liquids, developing jar, capillary tubes, and more, needed to perform the entire testing.Now included with every kit, Capillary tube extraction bulb, It makes it easier to take samples from reaction chambers.You get color charts from previous certified base line tests to match with your results and a THC % chart to match estimated % of THC and other chemicals. Represented as a chart of dots – each dot represents a % point. The larger the dot the more %, you compare the dots from your results to 

the ones on the chart; it is a basic chromatography way of 

testing substances.

Method of Testing using TLC Lab Supply Test Kits

TLC is a simple, quick, and inexpensive procedure that gives a quick answer as to how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf from the literature, In our case the cannabinoids can be identified by their specific color and specific location (Rf value) and order of appearance on the TLC plate, if carried out with the solvent system from the Kit.
A TLC plate is a sheet of glass which is coated with a thin layer of a solid adsorbent, silica. A small amount of the mixture to be analysed is spotted near the bottom of this plate. The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid, or eluent or carrier fluid, is the mobile phase, and it slowly rises up the 

TLC plate by capillary action.
As the solvent moves, it will pass the little spot of cannabinoid-oil micro-droplet of 2 ul or 4 ul that was applied on the bottom of the plate. An equilibrium is established for each component of the mixture between the molecules of that component which are absorbed on the solid and the molecules which are in solution. In principle, the components will differ in solubility and in strength of their adsorption to the adsorbent and the other components will be carried farther up to the plate than the others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried (ventilation!) . After drying, the separated components (cannabinoids) now separated in the silica plates, will be visualized by spraying the plate with a dye. The dye is highly specific for cannabinoids. Every principal cannabinoid will display his own color! THC: wine red, CBD: bright yellow, CBG: orange, CBN: violet etc.

Why HPTLC for botanical identification?

Other techniques can address identification only in part. They either are limited to plant parts (microscopy), have difficulties with natural variability of botanical materials (IR techniques), or focus on quantitative comparison of separated markers (HPLC).All other techniques lack the versatility and flexibility of HPTLC for the analysis of botanicals. HPTLC is the ONLY single technique that offers a generally applicable approach to compliance with the FDA requirement of 100% botanical identificationHPTLC is the primary chemical test for identification of all pharmacopoeia monographs on botanicals (including USP)The acceptance and performance of HPTLC is demonstrated by the China HPTLC Atlas, AHP monographs, and the USP Dietary Supplement CompendiumHPTLC fingerprints do not just give a correlation coefficient but visually show the similarities and differences between samples and references on the same plateIn addition to identity an HPTLC fingerprint can also give quantitative information (intensity of bands) about a sample for batch to batch comparisonHPTLC can also be used for assays (potency), purity (adulteration), and stability studies (shelf life)HPLC testing is more time consuming and have limited detectability of non UV absorbing compoundsUnlike HPLC, HPTLC preserves the complete sample for analysis, so nothing is "hidden". 


We are continuing to be the leader in the THC/CBD/CBN/CBG and Acids TLC testing industry. TLC Chromatography is the oldest documented technique to separate chemical substances and to record solubility of cannabinoid groups in flower. 


TLC Lab Supply train unbiased third-party individuals to asses THC/CBD/CBN/CBG and Acids percent of moisture, molds, fungus and test for pesticides. 


TLC Lab class assessment training certifies individuals seeking competency with 

CTK - TLC Test Kit procedures. 

Once you are certified you can begin to oversee the implementation process from start to finish.